Oct3/4 Antibody (DA191)

Description

POU5F1 (POU domain, class 5, transcription factor 1), also known as octamerbinding transcription factor-3 (Oct-3, Otf-3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.1-2 — Références — 1. Takeda, J., et al. 1992. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res. 20: 4613-4620. 2. Nichols, J., et al. 1998. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct-4. Cell 95: 379-391.

Caractéristiques

Type

Recombinant monoclonal rabbit antibody

Clone

DA191

Isotype

Rabbit

Réactivité

Synthetic peptide of human Oct3/4

Localisation

Nucleus

Usage prévu

IHC (FFPE)