MLH1 Antibody (DA081)

Description

MLH1 is a mismatch repair gene of around 87 kDa, commonly associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC). This gene was identified as a locus frequently mutated in HNPCC. It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively, spliced transcript variants encoding different isoforms have been described, but their fulllength natures have not been determined. In a high proportion of patients with microsatellite instability (MSI-H), the MLH1 protein is typically deficient. This protein deficiency is linked to the autosomal dominant condition of Hereditary Non-Polyposis Colon Cancer. The anti-MLH1antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite-unstable have a better prognosis than their microsatellite stable counterparts.1-6 — Références — 1. Wright CL, et al. Am J Surg Pathol. 2003;27:1393-1406 2. Brueckl WM, et al. Anticancer Research. 2003;23:1773-1778 3. Rigau V, et al. Arch Pathol Lab Med. 2003;127;June:694-700 4. Renkonen E, et al. J Clin Oncol. 2003;21:3629-3637 5. Hoedema R, et al. The American Surgeon. 2003;May69(5):387-92 6. U.S. Department of Health and Human Services: Centers for Disease Control and Prevention. Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories. Supplement / Vol. 61, January 6, 2012.

Caractéristiques

Type

Recombinant monoclonal mouse antibody

Clone

DA081

Isotype

Mouse

Réactivité

Synthetic peptide from human MLH1

Localisation

Nucleus

Usage prévu

IHC (FFPE)