MDM2 Antibody (DA080)

Description

Mouse double minute protein 2 (MDM2) is a gene encoded on the 12q13-14 chromosomal sequence.1-5 It encodes for a 483 amino acid residue protein which binds to the amino-terminal transcription region of p53.2,5 MDM2 has been shown to negatively regulate the tumor-suppressor activity of p53 by three mechanisms: Blocking p53 transcription, binding to p53 causing it to be exported from the nucleus, and accelerating the destruction of p53.1 MDM2 up-regulation has been shown in liposarcoma while being absent in lipoma.2,4 Therefore, anti-MDM2 has been demonstrated to be a potentially useful tool in distinguishing well-differentiated liposarcoma (atypical lipomatous tumor) from lipoma, with the neoplastic cells positive in the former lesion and negative in lipoma.2,4 — Références — 1. Uhrinova S, et al. Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding. J Mol Biol. 2005; 350:587-98. 2. Arici A, et al. Immunohistochemical detection of p53 and MDM2 expressions in liposarcoma with World health organization classification. Indian J Cancer. 2013; 50:164-9. 3. Ware PL, et al. MDM2 copy numbers in well-differentiated and dedifferentiated liposarcoma: characterizing progression to high-grade tumors. Am J Clin Pathol. 2014; 141:334-41. 4. Binh MB, et al. Reproducibility of MDM2 and CDK4 staining in soft tissue tumors. Am J Clin Pathol. 2006; 125:693-7. 5. Momand J, et al. The MDM2 gene amplification database. Nucleic Acids Res. 1998; 26:3453-9.

Caractéristiques

Type

Recombinant monoclonal mouse antibody

Clone

DA080

Isotype

Mouse

Réactivité

Synthetic peptide of human MDM2

Localisation

Nucleus

Usage prévu

IHC (FFPE)