Kappa Light Chain Antibody (DA075)

Description

Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, Kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulin. When studying B-cell neoplasms, the determination of light-chain ratios remains the centerpiece. This is sound reasoning because most B-cell Lymphomas express either the kappa light chains antibody or the lambda light chains antibody, whereas reactive proliferations display a mixture of kappa and lambda-positive cells. If only a single light-chain type is detected, a lympho-proliferative disorder is very likely. Monoclonality is determined by a kappa-lambda ratio greater than or equal to 3:1, a lambda-kappa ratio greater than or equal to 2:1, or a monoclonal population of 75% or more of the total population.1-5 — Références — 1. Michie SA et al. A J Clin Path. 1987 2. Hertel BF, et al. Lab Invest. 1977;36:12 3. Taylor CL, Arch Pathol Lab Med. 1978;12:113-121 4. Dogan A, Blood. 1998;91:4708-14 5. U.S. Department of Health and Human Services: Centers for Disease Control and Prevention. Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories. Supplement / Vol. 61, January 6, 2012. https://www.cdc.gov/mmwr/pdf/other/su6101.pdf

Caractéristiques

Type

Recombinant monoclonal mouse antibody

Clone

DA075

Isotype

Mouse

Réactivité

Synthetic peptide of human Kappa Light Chain

Localisation

Cytoplasm/Cell membrane

Usage prévu

IHC (FFPE)