Cyclin D1 Antibody (DA228)

Description

Detection of Cyclin D1 expression by immunohistochemistry (IHC) with Cyclin D1 (SP4) Rabbit Monoclonal Antibody may be used to aid in the diagnosis of mantle cell lymphomas. Cyclin D1 is a nuclear protein that is involved in regulation of the cell cycle.1 Cyclin D1 is normally expressed in the G1 (resting) phase of the cell cycle. In normal tissues, cyclin D1 expression by immunohistochemistry is restricted to epithelial tissues, endothelium and some fibroblasts and is not detectable in benign lymphocytes.2 Mutations in the cyclin D1 gene can lead to overexpression of cyclin D1 in lymphocytes and deregulation of the normal cell cycle, resulting in malignant transformation. Mantle cell lymphomas typically harbor a t(11;14) (q13;q32) translocation that juxtaposes the CCND1 (cyclin D1) gene with an enhancer of the IgH gene, resulting in cyclin D1 overexpression. Immunohistochemistry for cyclin D1 is positive in mantle cell lymphomas.1-3 Immunohistochemistry for cyclin D1 shows a nuclear pattern of staining. — Références — 1. Yatabe, Y et al. "Significance of cyclin D1 overexpression for the diagnosis of mantle cell lymphoma: a clinicopathologic comparison of cyclin D1-positive MCL and cyclin D1-negative MCL-like B-cell lymphoma." Blood vol. 95,7 (2000): 2253-61. 2. Cheuk, Wah et al. "Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody." The American journal of surgical pathology vol. 28,6 (2004): 801-7. 3. Izban, K F et al. "Multiparameter immunohistochemical analysis of the cell cycle proteins cyclin D1, Ki-67, p21WAF1, p27KIP1, and p53 in mantle cell lymphoma." Archives of pathology & laboratory medicine vol. 124,10 (2000): 1457-62.

Caractéristiques

Type

Recombinant monoclonal rabbit antibody

Clone

DA228

Isotype

Rabbit

Réactivité

Synthetic peptide of human Cyclin D1

Localisation

Nucleus

Usage prévu

IHC (FFPE)