CD45 Antibody (DA026)

Description

Leukocyte common antigens are a family of five or more high molecular weight glycoproteins that are located primarily on the surface of leukocytes, including T and B lymphocytes, polymorphonuclear leukocytes, and monocytes, and are not expressed in erythrocytes, platelets, or the nonhematopoietic system. Anti-CD45 (anti-leukocyte common antigen) is routinely used to aid the differential diagnosis of undifferentiated neoplasms, whenever malignant lymphoma is suspected by the morphological or clinical data. It is a highly specific antibody; therefore a positive result is highly indicative of hematolymphoid origin. Certain types of hematolymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.1-8 — Références — 1. Mason DY. A new look at lymphoma immunohistology. Am J Pathol. 1987; 128:1-4. 2. Hall PA, et al. Paraffin section immunohistochemistry. I. Non-Hodgkin's lymphoma. Histopathology. 1988; 13:149-60. 3. Kurtin PJ, et al.  Leukocyte common antigen--a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum Pathol. 1985; 16:353-65. 4. Maluf HM, et al. Fibroma and giant cell tumor of tendon sheath: a comparative histological and immunohistological study. Mod Pathol. 1995; 8:155-9. 5. Caballero T, et al. Intraepithelial and lamina propria leucocyte subsets in inflammatory bowel disease: an immunohistochemical study of colon and rectal biopsy specimens. J Clin Pathol. 1995; 48:743-8. 6. Vasef MA, et al. Immunophenotype of Reed-Sternberg and Hodgkin's cells in sequential biopsy specimens of Hodgkin's disease: a paraffin-section immunohistochemical study using the heat-induced epitope retrieval method. Am J Clin Pathol. 1997; 108:54-9. 7. Saxena, A et al. A combination of abnormal immunoarchitecture and reproducible clonal bands identifies the biologic nature of cutaneous B-cell infiltrates. Am J Clin Pathol 1999; 112:495-512. 8. Kraus, MD et al. Lymphocyte predominance Hodgkin's disease: the use of bcl-6 and CD57 in diagnosis and differential diagnosis. Am J Surg Pathol 2000; 24:1068-78.

Caractéristiques

Type

Recombinant monoclonal rabbit antibody

Clone

DA026

Isotype

Rabbit

Réactivité

Synthetic peptide of human CD45

Localisation

Cell membrane

Usage prévu

IHC (FFPE)