ATRX Antibody (DA273)

Description

Diffuse gliomas are classified based on histological and molecular features to achieve an integrated diagnosis. Molecular diagnostic markers include IDH mutation, 1p/19q co-deletion, and TP53 mutation. ATRX is a chromatin remodeling protein and its mutation status may be used as a molecular diagnostic marker within the diffuse glioma classification algorithm. Anti-ATRX is used to identify mutant ATRX by a loss of ATRX expression in neoplastic cells when compared with internal positive controls (endothelial cells, glia, and neurons). Grade II/III astrocytoma classification includes IDH mutant, ATRX mutant, and 1p/19q retention, while grade II/III oligodendroglioma includes IDH mutant, ATRX wildtype, and 1p/19q co-deletion; p53 expression may also serve as an aid in diagnosis. ATRX mutation is frequently, but not always, mutually exclusive with 1p/19q co-deletion.1-6 — Références — 1. Cancer Genome Atlas Research Network. Comprehensive, Integrative Genomic Analysis of Diffuse Lower-Grade Gliomas. N Engl J Med. 2015 Jun 25;372(26):2481-98. 2. Reuss, David E et al. "ATRX and IDH1-R132H immunohistochemistry with subsequent copy number analysis and IDH sequencing as a basis for an "integrated" diagnostic approach for adult astrocytoma, oligodendroglioma and glioblastoma." Acta neuropathologica vol. 129,1 (2015): 133-46. 3. Louis DN, et al. The 2016 World Health Organization Classification of Tumors of the Central Nervous System: a summary. Acta Neuropathol. 2016 Jun;131(6):803-20. 4. Ikemura, Masako et al. "Utility of ATRX immunohistochemistry in diagnosis of adult diffuse gliomas." Histopathology vol. 69,2 (2016): 260-7. 5. Yamamichi, Akane et al. "Immunohistochemical ATRX expression is not a surrogate for 1p19q codeletion." Brain tumor pathology vol. 35,2 (2018): 106-113. 6. Wood MD, et al. Applications of molecular neuro-oncology - a review of diffuse glioma integrated diagnosis and emerging molecular entities. Diagn Pathol. 2019 Apr 9;14(1):29.

Caractéristiques

Type

Recombinant monoclonal rabbit antibody

Clone

DA273

Isotype

Rabbit

Réactivité

Synthetic peptide of human ATRX

Localisation

Nucleus

Usage prévu

IHC (FFPE)