LEF-1 (EP310), RMab

Description

LEF-1 is a new rabbit monoclonal antibody for immunohistochemistry from Bio SB. Lymphoid enhancer-binding factor 1 (LEF1) is a protein that in humans is encoded by the LEF-1 gene with a 48-kD nuclear protein that is expressed in pre-B and T cells. LEF-1 coupling with β-catenin, functions as a key nuclear mediator of WNT/β-catenin signaling, which regulates cell proliferation and survival. LEF-1 has an important role in lymphopoiesis, and is normally expressed in T and pro-B cells but not mature B cells. LEF-1-mediated canonical Wnt signaling is required for morphogenesis of these skin appendages during embryogenesis. In normal lymphoid tissues, LEF-1 is nuclear localized and observed predominantly in T cells of the paracortical regions; staining was undetected in B cells. LEF-1 is highly overexpressed and associated with disease progression and poor prognosis in B-cell chronic lymphocytic leukemia. Strong nuclear expression of LEF-1 has been observed in majority of chronic lymphocytic leukemia/small lymphocytic lymphoma cases and LEF-1 is not detected in other small B cell lymphomas. Gene expression profiling revealed overexpression of LEF-1 in chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL). LEF-1 immunostaining has been detected in all neoplastic cells of CLL/SLL cases. LEF-1 was identified in 50% of high grade follicular lymphoma and 38% of diffuse large B-cell lymphoma, but not in mantle cell lymphoma or marginal zone lymphoma. Recently, high LEF-1 was demonstrated as a favorable prognostic marker in cytogenetically normal acute myeloid leukemia. Due to its high sensitivity, LEF-1 has been proposed to be a suitable immunohistochemical marker for diagnosis and differential diagnosis for CLL/SLL. Alternately spliced isoforms may play additional roles in regulating cell growth in colon carcinoma, and nuclear LEF-1 immunostaining was detected in 36% of adenocarcinoma brain metastases.

Caractéristiques

Type

Rabbit Monoclonal

Clone

EP310

Isotype

IgG

Réactivité

Paraffin, Frozen

Localisation

Nuclear

Contrôle

Breast, Tonsil, Breast Carcinoma, Small Lymphocytic Lymphoma

Usage prévu

For In Vitro Diagnostic Use